Research article summary (published 30 Oct 2009):

The impacts of ERCC1 gene exon VIII alternative splicing on cisplatin-resistance in ovarian cancer cells.

Full Abstract

Excision repair cross complementation group-1 (ERCC1) was reported to be responsible for drug resistance during cancer treatment. In this report, we first proved the existence of ERCC1 exon VIII alternative splicing in ovarian cancer cells. Further investigation showed that over-expressed exon VIII deficient ERCC1 variant failed to change the protein level of ERCC1 in cancer cells, but decreased the excision repair function of ERCC1 and enhanced sensitivity of cancer cells to cisplatin in a dose-dependent manner. The results indicate that ERCC1 exon VIII alternative splicing does exist in some ovarian cancer cell lines, and regulates cisplatin-resistance in ovarian cancer cells.

Author information

Author/s: Sun, Yehong (Y); Li, Tiyuan (T); Ma, Kewei (K); Tian, Zhongkai (Z); Zhu, Ying (Y); Chen, Fuqiang (F); Hu, Gang (G);

Affiliation: Department of Pharmacology, Nanjing Medical University, Nanjing, Jiangsu, PR China.

Journal and publication information

Publication Type: Journal Article

Journal: Cancer investigation (Cancer Invest), published in England. (Language: eng)

Reference: 2009-Nov; vol 27 (issue 9) : pp 891-7

Dates: Created 2009/10/16; Completed 2009/10/29;

PMID: 19832035, status: MEDLINE (last retrieval date: 10/29/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Alternative Splicing Antineoplastic Agents - pharmacology Cell Line, Tumor Cell Proliferation - drug effects Cell Survival - drug effects Cisplatin - pharmacology DNA Damage DNA-Binding Proteins - genetics; metabolism Dose-Response Relationship, Drug Drug Resistance, Neoplasm - genetics

Drug Resistance, Neoplasm - genetics Endonucleases - genetics; metabolism Exons Female Gene Expression Regulation, Enzymologic Gene Expression Regulation, Neoplastic Humans Ovarian Neoplasms - enzymology; genetics; pathology Reproducibility of Results Transfection

Associated Chemicals: Antineoplastic Agents (0) ; DNA-Binding Proteins (0) ; Cisplatin (15663-27-1) ; ERCC1 protein, human (EC 3.1.-) ; Endonucleases (EC 3.1.-)

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