Upregulated p27kip1 can downregulate survivin expression and inhibit telomerase activity in gastric carcinoma cells.

Full Abstract

Previous study indicated that p27kip1 gene transfer can obviously inhibit the growth of some carcinoma cells. The purpose of this study was to investigate anticarcinoma mechanism of p27kip1. Gastric carcinoma cell strain SGC-7901 was transfected with recombinant adenovirus vector carrying human p27kip1 gene (Ad-p27kip1). Expression of survivin was detected using the western blot method and activity of telomerase was examined by telomeric repeat amplification protocol (TRAP) PCR-ELISA in gastric carcinoma cells. Our results suggest that upregulated p27kip1 can downregulate survivin expression and inhibit telomerase activity in gastric carcinoma cells.

Author information

Author/s: Zhang, Weiguo (W); Tong, Qiang (Q); Wu, Qingming (Q); Li, Shengbao (S); Wang, Xiaohu (X); Wang, Qiang (Q);

Affiliation: Department of Gastroenterology, Taihe Hospital, Yunyang Medical College, Shiyan, Hubei, China.

Journal and publication information

Publication Type: Journal Article

Journal: Cancer investigation (Cancer Invest), published in England. (Language: eng)

Reference: 2009-Nov; vol 27 (issue 9) : pp 898-900

Dates: Created 2009/10/16; Completed 2009/10/29;

PMID: 19832036, status: MEDLINE (last retrieval date: 10/29/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Intracellular Signaling Peptides and Proteins - genetics; metabolism
Microtubule-Associated Proteins - metabolism
Polymerase Chain Reaction
Stomach Neoplasms - enzymology; genetics; pathology
Telomerase - antagonists & inhibitors; genetics; metabolism
Time Factors
Transfection
Up-Regulation

Associated Chemicals: BIRC5 protein, human (0) ; CDKN1B protein, human (0) ; Intracellular Signaling Peptides and Proteins (0) ; Microtubule-Associated Proteins (0) ; Telomerase (EC 2.7.7.49)

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