Enhanced MDR1 expression and chemoresistance of cancer stem cells derived from glioblastoma.

Full Abstract

We established a cancer stem (CS) cell line, U87CS, by means of spheroid culture of U87MG cells derived from glioblastoma (GBM) in neuronal stem cell medium. U87CS cells presented positive immunohistochemical staining for multidrug resistance (MDR)1 and CD133, a marker for a subset of leukemia and GBM CS cells. The gene expression of MDR1 and CD133 on U87CS cells increased by an average of 8.51 and 47.18 times, respectively, compared to the levels on U87MG cells by real-time quantitative RT-PCR. U87CS cells possessed stronger drug-resistance to conventional anti-cancer drugs, such as doxorubicin (Dox), etoposide (VP-16), carboplastin, and BCNU than U87MG cells. Double immunofluoresence staining showed co-expression of MDR1 and CD133 on U87CS cells transplanted into nude mice brains. In addition, we identified the crossreactivity of CD133 and MDR1 in a surgical specimen of GBM. Our results suggest that CS cells may be resistant to current chemotherapy and represent a novel target for GBM therapeutics.

Author information

Author/s: Nakai, Eiichi (E); Park, Kaechang (K); Yawata, Toshio (T); Chihara, Takahiro (T); Kumazawa, Ayano (A); Nakabayashi, Hiromichi (H); Shimizu, Keiji (K);

Affiliation: Department of Neurosurgery, Medical School, Kochi University, Kochi, Japan.

Journal and publication information

Publication Type: Journal Article

Journal: Cancer investigation (Cancer Invest), published in England. (Language: eng)

Reference: 2009-Nov; vol 27 (issue 9) : pp 901-8

Dates: Created 2009/10/16; Completed 2009/10/29;

PMID: 19832037, status: MEDLINE (last retrieval date: 10/29/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Glycoproteins - genetics; metabolism
Humans
Male
Mice
Mice, Nude
Neoplastic Stem Cells - drug effects; metabolism; pathology
P-Glycoprotein - genetics; metabolism
Peptides - genetics; metabolism
Reverse Transcriptase Polymerase Chain Reaction
Spheroids, Cellular
Up-Regulation

Associated Chemicals: ABCB1 protein, human (0) ; AC133 antigen (0) ; Antigens, CD (0) ; Antineoplastic Agents (0) ; Glycoproteins (0) ; P-Glycoprotein (0) ; Peptides (0)

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