Research article summary (published 30 Oct 2009):

Effect of ibuprofen on osteoblast differentiation of porcine bone marrow-derived progenitor cells.

Full Abstract

PURPOSE: Nonsteroidal anti-inflammatory drugs are commonly prescribed to reduce inflammation and pain. However, little is known about the direct effect of these drugs on the differentiation of bone marrow-derived progenitor cells into osteoblasts. The purpose of this study was to determine the effect of ibuprofen on osteoblast differentiation and proliferation in a minipig model. MATERIALS AND METHODS: Bone marrow was aspirated from the minipig ilium, and porcine bone marrow-derived progenitor cells (pBMPCs) were isolated and expanded in standard culture medium. The pBMPCs were replated and differentiated into osteoblasts by use of osteogenic supplements (OS). Five groups were studied: negative control--pBMPCs in standard medium only; positive control--pBMPCs, standard culture medium, and OS; and 3 experimental groups--pBMPCs, standard culture medium, OS, and ibuprofen added in doses of 0.1, 1.0, and 3.0 mmol/L. Cell cultures were evaluated quantitatively by alkaline phosphatase (ALP) stain, von Kossa stain, and deoxyribonucleic acid (DNA) content. RESULTS: pBMPCs cultured with OS and low-dose ibuprofen (0.1 mmol/L) showed ALP stain, von Kossa stain, and DNA content similar to pBMPCs cultured in OS (positive control). pBMPCs cultured in higher doses of ibuprofen (1.0 and 3.0 mmol/L) produced significantly less positive staining of ALP and von Kossa and decreased DNA content. CONCLUSION: The results indicate that high-dose ibuprofen has a deleterious effect on pBMPC differentiation into osteoblasts whereas low-dose ibuprofen does not. The low dose of 0.1 mmol/L is the typical serum level when prescribed for clinical use.

Author information

Author/s: Abukawa, Harutsugi (H); Phelps, Maynard (M); Jackson, Pamela (P); Smith, R Malcolm (RM); Vacanti, Joseph P (JP); Kaban, Leonard B (LB); Troulis, Maria J (MJ);

Affiliation: Department of Oral and Maxillofacial Surgery, Tokyo Medical University, Tokyo, Japan.

Journal and publication information

Publication Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't

Journal: Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons (J Oral Maxillofac Surg), published in United States. (Language: eng)

Reference: 2009-Nov; vol 67 (issue 11) : pp 2412-7

Dates: Created 2009/10/19; Completed 2009/11/03;

PMID: 19837310, status: MEDLINE (last retrieval date: 11/3/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

External Links for this article
(including full text providers, if available):

Click Electronic Full-text Provider Links to see options for finding the electronic full text links to this article. Note there may be a subscription or fee required for access to the full text. See our FAQ for information on finding FREE full text articles.

This article may also be located in paper journal collections available in many libraries. Use the Journal and Publication Information above to find the full article.

MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Alkaline Phosphatase - metabolism Animals Anti-Inflammatory Agents, Non-Steroidal - pharmacology Bone Marrow Cells - cytology; drug effects; enzymology Cell Differentiation - drug effects Cells, Cultured Dose-Response Relationship, Drug

Dose-Response Relationship, Drug Female Ibuprofen - pharmacology Mesenchymal Stem Cells - cytology; drug effects; enzymology Osteoblasts - cytology; drug effects; enzymology Swine Swine, Miniature

Associated Chemicals: Anti-Inflammatory Agents, Non-Steroidal (0) ; Ibuprofen (15687-27-1) ; Alkaline Phosphatase (EC 3.1.3.1)

Related articles

These are the highest related articles currently in the database:

• Stimulatory effects of statins on bone marrow-derived mesenchymal stem cells. Study of a new therapeutic agent for fracture. 30 Dec 2004
• Tigogenin inhibits adipocytic differentiation and induces osteoblastic differentiation in mouse bone marrow stromal cells. 10 Feb 2007
• The effect of purmorphamine on osteoblast phenotype expression of human bone marrow mesenchymal cells cultured on titanium. 29 Jun 2005
• The effects of IGF-I and IGF-II on proliferation and differentiation of human osteoblasts and interactions with growth hormone. 27 Feb 1998
• Antibacterial and osteogenic properties of silver-containing hydroxyapatite coatings produced using a sol gel process. 13 Sep 2007
• Prostaglandins differentially affect osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells. 29 Sep 2007
• [Inducing human marrow mesenchymal stem cells into osteoblasts directionally and identification of their osteogenesis characteristics] 28 Feb 2004
• Enhancement of osteoblastic differentiation of mesenchymal stromal cells cultured by selective combination of bone morphogenetic protein-2 (BMP-2) and fibroblast growth factor-2 (FGF-2). 29 Jun 2007
• Osteogenic growth peptide C-terminal pentapeptide [OGP(10-14)] acts on rat bone marrow mesenchymal stem cells to promote differentiation to osteoblasts and to inhibit differentiation to adipocytes. 25 Jan 2007
• Effects of continuous dexamethasone treatment on differentiation capabilities of bone marrow-derived mesenchymal cells. 8 Jul 2007

See 100+ related articles.

Related Article Map

Legend: - FREE Full text Article. - Abstract only. - Title only. More help.

See a large map of 100+ related articles.