Heterotransplantation of canine sarcoma cells (BLU284) in athymic (nude) mice.

Full Abstract

Undifferentiated canine sarcoma cells from a primary lesion were implanted subcutaneously in athymic (nude) mice. Tumors were implanted at 2 sites in each of 4 mice, and 6 of the 8 inoculation sites developed into tumors. Tumors grew and invaded surrounding tissues, as shown by histologic examination. Karyotype analysis verified that tumors were of canine origin. Cells from this tumor were serially heterotransplanted 26 times without consistent change in growth rate. Once established in nude mice, samples from 2 generations of this canine sarcoma cell line were stored cryogenically and were implanted into nude mice. Over 26 generations, 93.7% of fresh implants developed into tumors. Preliminary screening of antineoplastic drugs indicated that this tumor line was sensitive to cyclophosphamide and vincristine. The difference in sensitivity of this heterotransplanted tumor in nude mice toward the various antineoplastic agents provides a useful model for the elucidation of biochemical bases of antineoplastic drug resistance in tumors.

Author information

Author/s: Aaronson, L M (LM); Pardini, L L (LL); Pardini, R S (RS);

Journal and publication information

Publication Type: Journal Article

Journal: American journal of veterinary research (Am J Vet Res), published in UNITED STATES. (Language: eng)

Reference: 1987-May; vol 48 (issue 5) : pp 816-21

Dates: Created 1987/07/09; Completed 1987/07/09; Revised 2003/11/14;

PMID: 3592383, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

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