Research article summary (published 27 Feb 1983):

Effect of lanthanum on pancreatic protein synthesis in streptozotocin-diabetic rats.

Full Abstract

The role of extracellular Ca2+ in mediating the stimulatory effect of cholecystokinin octapeptide (CCK8) on [3H]phenylalanine incorporation into protein was studied in isolated pancreatic acini from streptozotocin-diabetic rats. The stimulatory effect of CCK8 (10(-10) M) on [3H]phenylalanine incorporation was completely abolished by preincubating acini with either 10(-4) M lanthanum or 10(-3) M manganese. At these concentrations neither compound altered the basal rate of amino acid incorporation, and both compounds inhibited CCK-mediated Ca2+ influx without affecting either basal or CCK-mediated 45Ca2+ efflux. Lanthanum (10(-4) M) also blocked the stimulatory effect of the cholinergic analogue carbachol (10(-5) M) on amino acid incorporation but did not alter the stimulatory effect of insulin (1.67 x 10(-8) M). Vasoactive intestinal polypeptide and 12-O-tetradecanoyl-phorbol-13-acetate failed to increase the incorporation of [3H]phenylalanine into acinar protein. These findings suggest that CCK and other pancreatic secretagogues that act via Ca2+ enhance protein synthesis by increasing cell membrane permeability to Ca2+ and provide additional evidence that this may be an important mechanism by which CCK regulates pancreatic exocrine function.

Author information

Author/s: Korc, M (M);

Grants: AM-28313 (Agency:NIADDK NIH HHS)

Journal and publication information

Publication Type: Journal Article; Research Support, U.S. Gov't, P.H.S.

Journal: The American journal of physiology (Am J Physiol), published in UNITED STATES. (Language: eng)

Reference: 1983-Mar; vol 244 (issue 3) : pp G321-6

Dates: Created 1983/04/15; Completed 1983/04/15; Revised 2007/11/14;

PMID: 6299118, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Animals Biological Transport - drug effects Calcium - metabolism Carbachol - pharmacology Cholecystokinin - pharmacology Cycloleucine - metabolism Diabetes Mellitus - metabolism Insulin - pharmacology Islets of Langerhans - drug effects; metabolism Kinetics

Kinetics Lanthanum - pharmacology Male Manganese - pharmacology Peptide Fragments - pharmacology Phenylalanine - metabolism Protein Biosynthesis Rats Rats, Inbred Strains Sincalide

Associated Chemicals: Peptide Fragments (0) ; Insulin (11061-68-0) ; Sincalide (25126-32-3) ; Carbachol (51-83-2) ; Cycloleucine (52-52-8) ; Phenylalanine (63-91-2) ; Lanthanum (7439-91-0) ; Manganese (7439-96-5) ; Calcium (7440-70-2) ; Cholecystokinin (9011-97-6)

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