Antisense oligonucleotide inhibits the expression of procollagen type III gene in mouse fibroblasts.

Full Abstract

The effects of transfection of phosphorothioate antisense oligonucleotides (ASOs) into BALB/c 3T3 mouse fibroblasts on procollagen type III production were examined. Immunohistochemistry and Western blots showed that the expression of procollagen type III decreased. Semi-quantitative reverse transcription polymerase chain reaction revealed that the expression of procollagen type III mRNA was inhibited. ASOs were effective for inhibiting procollagen type III production at the gene level, although the effect was transient.

Author information

Author/s: Kitamura, Y (Y); Tanigawa, T (T); Nanba, E (E); Ueno, E (E); Fukumoto, S (S); Ichihara, K (K);

Affiliation: Second Department of Pathology, Tottori University Faculty of Medicine, Yonago, Japan.

Journal and publication information

Publication Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't

Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun), published in UNITED STATES. (Language: eng)

Reference: 1995-Oct; vol 215 (issue 3) : pp 849-54

Dates: Created 1995/11/29; Completed 1995/11/29; Revised 2006/11/15;

PMID: 7488051, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

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