Research article summary (published 30 Dec 1992):

Relationship between the structure and function of Escherichia coli initiator tRNA.

Full Abstract

Through functional studies of mutant tRNAs, we have identified sequence and/or structural features important for specifying the many distinctive properties of E coli initiator tRNA. Many of the mutant tRNAs contain an anticodon sequence change from CAU-->CUA and are now substrates for E coli glutaminyl-tRNA synthetase (GlnRS). We describe here the effect of further mutating the discriminator base 73 and nucleotide 72 at the end of the acceptor stem on: i) recognition of the mutant tRNAs by E coli GlnRS; ii) recognition by E coli methionyl-tRNA transformylase; and iii) activity of the mutant tRNAs in initiation in E coli. For GlnRS recognition, our results are, in general, consistent with interactions found in the crystal structure of the E coli GlnRS-glutamine tRNA complex. The results also support our previous conclusion that formylation of initiator tRNA is important for its function in initiation.

Author information

Author/s: Dyson, M R (MR); Mandal, N (N); RajBhandary, U L (UL);

Affiliation: Institute of Cell and Molecular Biology, University of Edinburgh, UK.

Grants: GM17151 (Agency:NIGMS NIH HHS)

Journal and publication information

Publication Type: Journal Article; Research Support, U.S. Gov't, P.H.S.

Journal: Biochimie (Biochimie), published in FRANCE. (Language: eng)

Reference: 1993-; vol 75 (issue 12) : pp 1051-60

Dates: Created 1994/07/07; Completed 1994/07/07; Revised 2007/11/14;

PMID: 7515283, status: MEDLINE (last retrieved date: 2/18/2009)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MeSH Headings (categories) shown below.

Acyltransferases - chemistry; genetics; metabolism Amino Acyl-tRNA Synthetases - chemistry; genetics; metabolism Base Sequence Binding Sites Escherichia coli - chemistry; genetics; metabolism Hydroxymethyl and Formyl Transferases Immunoblotting Molecular Sequence Data

Mutation Nucleic Acid Conformation Peptide Chain Initiation, Translational RNA, Bacterial - chemistry; genetics; metabolism RNA, Transfer, Amino Acyl - chemistry; genetics; metabolism RNA, Transfer, Met Structure-Activity Relationship Substrate Specificity

Note: Bold headings indicate primary MeSH headings or qualifiers.

Associated Chemicals: RNA, Bacterial (0) ; RNA, Transfer, Amino Acyl (0) ; RNA, Transfer, Met (0) ; tRNA, formylmethionine- (0) ; Hydroxymethyl and Formyl Transferases (EC 2.1.2.-) ; methionyl-tRNA formyltransferase (EC 2.1.2.9) ; Acyltransferases (EC 2.3.-) ; Amino Acyl-tRNA Synthetases (EC 6.1.1.-) ; glutaminyl-tRNA synthetase (EC 6.1.1.18)

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