In vitro allergic bronchoconstriction in the brown Norway rat.

Full Abstract

The ovalbumin (OA)-sensitized Brown Norway rat (BN) demonstrates early-response (ER) and late-response (LR) allergic bronchoconstriction. To determine whether these responses could be replicated in vitro, we studied lung explants from 8-wk-old male BN rats (wt: 239 +/- 28 g), of which 19 were sensitized to OA (test) and 16 served as controls. Two weeks after sensitization, the animals' lungs were removed, filled with a 1% (wt/vol) agarose-containing solution at 37 degrees C, and cooled to 4 degrees C. Transverse slices (0.5 to 1.0 mm thick) were cut and cultured overnight. Airways were visualized with an inverted microscope and baseline images were obtained with a video camera. To study the ER, 40 airways from 15 test rats and 29 airways from 10 control rats were challenged with 2 micrograms OA and imaged each minute for 10 min. To study the LR, 40 airways from 12 test rats and 44 airways from 12 control rats were challenged with 2 micrograms OA and imaged each hour for 8 h. The maximal response (MR) for each airway was defined as the percent of airway closure. The ER and LR were both defined as an MR > or = mean + 2 SD of the controls. An ER occurred in 38 of 40 test and 2 of 29 control airways (mean MR: 42 +/- 24% versus 4 +/- 3%, p < 0.001), and was completely blocked by methysergide pretreatment in 13 airways.(ABSTRACT TRUNCATED AT 250 WORDS)

Author information

Author/s: Dandurand, R J (RJ); Wang, C G (CG); Laberge, S (S); Martin, J G (JG); Eidelman, D H (DH);

Affiliation: Meakins-Christie Laboratories, Royal Victoria Hospital, Montreal, Quebec, Canada.

Journal and publication information

Publication Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't

Journal: American journal of respiratory and critical care medicine (Am J Respir Crit Care Med), published in UNITED STATES. (Language: eng)

Reference: 1994-Jun; vol 149 (issue 6) : pp 1499-505

Dates: Created 1994/07/15; Completed 1994/07/15; Revised 2006/11/15;

PMID: 8004305, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Airway Resistance
Animals
Asthma - etiology; pathology; physiopathology
Bronchial Provocation Tests
Bronchodilator Agents - immunology
Constriction, Pathologic - etiology; pathology; physiopathology
Disease Models, Animal
Drug Hypersensitivity - blood; complications; immunology; physiopathology
Hypersensitivity, Delayed - blood; complications; immunology; physiopathology
Hypersensitivity, Immediate - blood; complications; immunology; physiopathology
Immunoglobulin E - blood

Immunoglobulin E - blood
Leukotriene D4 - antagonists & inhibitors; immunology
Male
Methysergide - immunology
Ovalbumin
Premedication
Propionates - immunology
Quinolines - immunology
Rats
Serotonin - immunology
Time Factors

Associated Chemicals: Bronchodilator Agents (0) ; Propionates (0) ; Quinolines (0) ; verlukast (115104-28-4) ; Methysergide (361-37-5) ; Immunoglobulin E (37341-29-0) ; Serotonin (50-67-9) ; Leukotriene D4 (73836-78-9) ; Ovalbumin (9006-59-1)

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