Research article summary (published 30 May 1993):

Evidence for a novel glycinamide ribonucleotide transformylase in Escherichia coli.

Full Abstract

We demonstrate here that Escherichia coli synthesizes two different glycinamide ribonucleotide (GAR) transformylases, both catalyzing the third step in the purine biosynthetic pathway. One is coded for by the previously described purN gene (GAR transformylase N), and a second, hitherto unknown, enzyme is encoded by the purT gene (GAR transformylase T). Mutants defective in the synthesis of the purN- and the purT-encoded enzymes were isolated. Only strains defective in both genes require an exogenous purine source for growth. Our results suggest that both enzymes may function to ensure normal purine biosynthesis. Determination of GAR transformylase T activity in vitro required formate as the C1 donor. Growth of purN mutants was inhibited by glycine. Under these conditions GAR accumulated. Addition of purine compounds or formate prevented growth inhibition. The regulation of the level of GAR transformylase T is controlled by the PurR protein and hypoxanthine.

Author information

Author/s: Nygaard, P (P); Smith, J M (JM);

Affiliation: Institute of Biological Chemistry B, University of Copenhagen, Denmark.

Grants: AI20068 (Agency:NIAID NIH HHS)

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.

Journal: Journal of bacteriology (J Bacteriol), published in UNITED STATES. (Language: eng)

Reference: 1993-Jun; vol 175 (issue 11) : pp 3591-7

Dates: Created 1993/07/01; Completed 1993/07/01; Revised 2008/11/20;

PMID: 8501063, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Acyltransferases - analysis; genetics Amino Acids - pharmacology Escherichia coli - drug effects; enzymology; genetics Escherichia coli Proteins Formates - metabolism Gene Expression Regulation, Bacterial

Genes, Bacterial - genetics Glycine - metabolism Hydroxymethyl and Formyl Transferases Isoenzymes - genetics Phosphoribosylglycinamide Formyltransferase Purines - metabolism

Associated Chemicals: Amino Acids (0) ; Escherichia coli Proteins (0) ; Formates (0) ; Isoenzymes (0) ; Purines (0) ; Glycine (56-40-6) ; Hydroxymethyl and Formyl Transferases (EC 2.1.2.-) ; purT protein, E coli (EC 2.1.2.-) ; Phosphoribosylglycinamide Formyltransferase (EC 2.1.2.2) ; Acyltransferases (EC 2.3.-)

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