Expression and release of interleukin-1 by human glioblastoma cells in vitro and in vivo.

Full Abstract

The present study demonstrates interleukin-1 (IL-1) production by human glioblastoma cells both in vitro and in vivo. The presence of IL-1 alpha and IL-1 beta transcripts was analyzed in 4 cell lines. IL-1 alpha mRNA was expressed constitutively in one cell line whereas constitutive IL-1 beta mRNA could not be detected in any of the cell lines. IL-1 alpha transcripts could be induced with phorbol myristate acetate (PMA) or PMA plus lipopolysaccharide (LPS) in 2 of 4 cell lines and IL-1 beta mRNA in 2 of 4 cell lines. Culture fluid from these cell lines was tested for the presence of IL-1 using a specific radio-immuno-assay for either IL-1 alpha or IL-1 beta. In agreement with the results on RNA, one of 4 cell lines was found to constitutively produce IL-1 alpha but not IL-1 beta. After treatment with PMA and LPS, IL-1 alpha was detected in the culture fluid from two other lines and IL-1 beta in the medium from three lines. That the IL-1 produced by these cell lines was biologically active was confirmed in a two step thymocyte proliferation assay. IL-1 like activity was detected in all samples that were positive in the radio-immuno-assay. Finally, immunohistological analysis on fresh frozen tumour sections provided evidence for IL-1 production by glioblastoma cells in vivo. Fourteen out of 28 glioblastomas were stained with an anti-IL-1 alpha monoclonal antibody while none of them was stained with an anti-IL-1 beta antibody.

Author information

Author/s: Gauthier, T (T); Hamou, M F (MF); Monod, L (L); Gallay, P (P); Carrel, S (S); de Tribolet, N (N);

Affiliation: Neurosurgical Service, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland.

Journal and publication information

Publication Type: Journal Article

Journal: Acta neurochirurgica (Acta Neurochir (Wien)), published in AUSTRIA. (Language: eng)

Reference: 1993-; vol 121 (issue 3-4) : pp 199-205

Dates: Created 1993/07/15; Completed 1993/07/15; Revised 2004/11/17;

PMID: 8512018, status: MEDLINE (last retrieved date: 2/18/2009)

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