Clinico-pathological studies on the effect of different anti-neoplastic chemotherapy regimens on transmissible venereal tumours in dogs.

Full Abstract

Thirty-two dogs affected with transmissible venereal tumour (TVT) were divided into three treatment groups. In group I vincristine sulphate at 0.025 mg/kg body weight, in group II vinblastine sulphate at 0.150 mg/kg body weight, and in group III vinblastine sulphate at 0.100 mg/kg body weight plus methotrexate at 0.35 mg/kg body weight were given intravenously at weekly intervals. Biopsies were performed on days 0, 3, 7 and 14. The tissues were preserved in 10% neutral buffered formalin and processed routinely for haematoxylin and eosin staining. Histopathologically, the untreated TVT was characterized by sheets or bundles of mostly rounded cells having a large, highly basophilic nucleus with a prominent, highly basophilic necleolus. Both vincristine and vinblastine primarily affected the nuclei of neoplastic cells, causing condensation, karyorrhexis and karyolysis within 3 days of chemotherapy. The regressing tumour mass showed marked infiltration by lymphocytes, lymphoblasts and macrophages by day 7. There was nearly complete regression of the tumour by day 14, as shown by the almost complete loss of neoplastic cells, with fibrous tissue substitution. However, in group III, the changes occurred more slowly and more injections were needed for complete regression. In both groups I and II, 11/12 of the animals responded completely to the chemotherapy within 3 weeks, while in group III, 6/8 of the dogs responded to the treatment by 21-28 days.

Author information

Author/s: Singh, J (J); Rana, J S (JS); Sood, N (N); Pangawkar, G R (GR); Gupta, P P (PP);

Affiliation: Department of Gynaecology and Obstetrics, College of Veterinary Science, Punjab Agricultural University, Ludhiana, India.

Journal and publication information

Publication Type: Comparative Study; Journal Article

Journal: Veterinary research communications (Vet Res Commun), published in NETHERLANDS. (Language: eng)

Reference: 1996-; vol 20 (issue 1) : pp 71-81

Dates: Created 1996/08/27; Completed 1996/08/27; Revised 2006/11/15;

PMID: 8693703, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

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