Differential action of branching enzymes according to its source.

Full Abstract

Different branching enzymes were partially purified in order to get some insight into their mechanism of action. Each branching enzyme is able to synthesize in vitro at any moment, even during very short incubation times, only one type of alpha 1,4-alpha 1,6 glucopolysaccharide, which possesses unique and specific properties (lambda max; A; R;% of beta-amylolysis;% alpha 1,6). Between two branching points, each branching enzyme will leave, in average, a constant and specific number of glucoses alpha 1,4-linked, which is characteristic of that particular enzyme. Consequently the structure of the polysaccharide will keep the same independently from the moment of synthesis.

Author information

Author/s: Lerner, L R (LR); Krisman, C R (CR);

Affiliation: Instituto de Investigaciones Bioquímicas Luis F. Leloir Fundación Campomar, Argentina.

Journal and publication information

Publication Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't

Journal: Cellular and molecular biology (Noisy-le-Grand, France) (Cell Mol Biol (Noisy-le-grand)), published in FRANCE. (Language: eng)

Reference: 1996-Jul; vol 42 (issue 5) : pp 599-608

Dates: Created 1997/01/08; Completed 1997/01/08; Revised 2006/11/15;

PMID: 8832089, status: MEDLINE (last retrieved date: 2/18/2009)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MeSH Headings (categories) shown below.

1,4-alpha-Glucan Branching Enzyme - isolation & purification; metabolism
Animals
Carbohydrate Conformation
Glucans - biosynthesis; chemistry
Glucose - chemistry; metabolism
Glycogen - biosynthesis; chemistry
Liver - enzymology

Liver - enzymology
Models, Biological
Polysaccharides - biosynthesis; chemistry
Rats
Solanum tuberosum - enzymology
Spectrophotometry
Zea mays - enzymology

Note: Bold headings indicate primary MeSH headings or qualifiers.

Associated Chemicals: Glucans (0) ; Polysaccharides (0) ; Glucose (50-99-7) ; Glycogen (9005-79-2) ; 1,4-alpha-Glucan Branching Enzyme (EC 2.4.1.18)

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