Interleukin-1 alpha produced in human gingival fibroblasts induces several activities related to the progression of periodontitis by direct contact.

Full Abstract

Previous observations suggest that interleukin-1 (IL-1) may play an important role in the progression of periodontitis. In the present study, we investigated whether a cell-associated IL-1 alpha (CAIL-1 alpha) produced in human gingival fibroblasts (HGF) induces biological activities related to the progression of periodontitis. HGF were treated with recombinant human IL-1 beta (rhIL-1 beta) for 12 h. After that, the cell layers of HGF were washed 3 times with fresh medium and were then fixed with 1% paraformaldehyde. The fixed cell layers of HGF were used for assays for bone resorbing activity, prostaglandin E2 (PGE2) production and collagenase activity. Fixed cell layers of HGF treated with rhIL-1 beta enhanced not only calcium release from BALB/c mouse calvaria but also PGE2 production and collagenase activity in HGF and human periodontal ligament fibroblasts (HPLF) cultured on the fixed cell layers. These activities were neutralized by treatment with monoclonal mouse anti-human IL-1 alpha antibody, but monoclonal mouse anti-human IL-1 beta antibody showed no effects on these activities. The induction of these activities by fixed cell layers of HGF required direct contact between the fixed cell layers and the calvaria, HGF, or HPLF. These results suggest that CAIL-1 alpha produced in HGF treated with rhIL-1 beta induces bone resorbing activity, PGE2 production and collagenase activity in the target cells by direct contact; CAIL-1 alpha may play an important role in the progression of periodontitis.

Author information

Author/s: Okamatsu, Y (Y); Kobayashi, M (M); Nishihara, T (T); Hasegawa, K (K);

Affiliation: Department of Periodontics, Showa University, Dental School, Tokyo, Japan.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Journal of periodontal research (J Periodontal Res), published in DENMARK. (Language: eng)

Reference: 1996-Jul; vol 31 (issue 5) : pp 355-64

Dates: Created 1996/12/19; Completed 1996/12/19; Revised 2006/11/15;

PMID: 8858540, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

External Links for this article
(including full text providers, if available):

Click Electronic Full-text Provider Links to see options for finding the electronic full text links to this article. Note there may be a subscription or fee required for access to the full text. See our FAQ for information on finding FREE full text articles.

This article may also be located in paper journal collections available in many libraries. Use the Journal and Publication Information above to find the full article.

MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Fibroblasts - drug effects; enzymology; immunology; metabolism
Gingiva - cytology; drug effects; immunology; metabolism
Humans
Interleukin-1 - biosynthesis; pharmacology; physiology
Mice
Periodontal Ligament - immunology; metabolism; physiology
Periodontitis - immunology; physiopathology
Recombinant Proteins - pharmacology

Associated Chemicals: Antibodies, Monoclonal (0) ; Interleukin-1 (0) ; Recombinant Proteins (0) ; Dinoprostone (363-24-6) ; Collagenases (EC 3.4.24.-)

These are the highest related articles currently in the database: