[Contribution of flow cytometry to allergologic diagnosis]

(Apport de la cytométrie en flux dans le diagnostic allergologique.)

Full Abstract

The technique of Flow Cytometry for activation of basophils (TAB), expressed as the marker CD63, is at present one of the pathways of research applied to drug allergy. In this work are reported the results of TAB of Pneumoallergens and Hymenoptera venoms. TAB can define better than total IgE the atopy of the subject: in effect the fluorescence of the basophils is emphasized in comparison with non-atopic subjects. This hypothesis has been confirmed by a study of three groups of subjects. With regard to drug allergy, it is important to study patients in whom the observations have been documented very objectively by clinical history and positive skin tests to the drug, compared with a negative reference to the same drug. So, TAB has been shown to be very useful in diagnosis of allergy to certain drugs, such as the Myorelaxants.

Author information

Author/s: Sabbah, A (A); Drouet, M (M); Sainte-Laudry, J (J); Lauret, M G (MG); Loiry, M (M);

Affiliation: Laboratoire d'Immuno-Allergologie-CHU Angers.

Journal and publication information

Publication Type: Comparative Study; English Abstract; Journal Article

Journal: Allergie et immunologie (Allerg Immunol (Paris)), published in FRANCE. (Language: fre)

Reference: 1997-Jan; vol 29 (issue 1) : pp 15-21

Dates: Created 1997/07/08; Completed 1997/07/08; Revised 2006/11/15;

PMID: 9213415, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

External Links for this article
(including full text providers, if available):

Click Electronic Full-text Provider Links to see options for finding the electronic full text links to this article. Note there may be a subscription or fee required for access to the full text. See our FAQ for information on finding FREE full text articles.

This article may also be located in paper journal collections available in many libraries. Use the Journal and Publication Information above to find the full article.

MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Allergens - diagnostic use; immunology
Anaphylaxis - chemically induced; diagnosis; immunology
Androstanols - adverse effects; immunology
Animals
Antigens, CD - analysis
Basophils - drug effects; immunology
Bee Venoms - immunology
Dogs
Drug Hypersensitivity - diagnosis; immunology
Dust

Flow Cytometry
Histamine Release
Hypersensitivity, Immediate - diagnosis; immunology
Immunoglobulin E - analysis; immunology
Mites - immunology
Neuromuscular Nondepolarizing Agents - adverse effects; immunology
Platelet Membrane Glycoproteins - analysis
Pollen - immunology
Skin Tests
Wasp Venoms - immunology

Associated Chemicals: Allergens (0) ; Androstanols (0) ; Antigens, CD (0) ; Bee Venoms (0) ; Dust (0) ; Neuromuscular Nondepolarizing Agents (0) ; Platelet Membrane Glycoproteins (0) ; Wasp Venoms (0) ; lysosomal protein GP53 (0) ; rocuronium (143558-00-3) ; Immunoglobulin E (37341-29-0)

These are the highest related articles currently in the database: