Sulfidoleukotriene release test (CAST) in hypersensitivity to nonsteroidal anti-inflammatory drugs.

Full Abstract

There is a great need to develop a method for making an accurate and reliable in vitro diagnosis of adverse hypersensitivity reactions to drugs. We measured the amount of sulfidoleukotriene (sLT) released from the peripheral blood leukocytes obtained from 25 patients who developed hypersensitivity reactions following the administration of nonsteroidal anti-inflammatory drugs (NSAIDs); 12 patients demonstrated reactions to Voltaren, 8 patients to Bufferin, and 5 patients to Sedes G. The stimulation index, the ratio of the amounts of sLT (pg/ml) incubated with and without drugs, was considerably higher in the patients than in the controls, which consisted of 5 nonallergic healthy subjects. The sensitivity of the CAST (cellular antigen stimulation test) was evaluated to range from 62.5 to 80%, while the specificity was 70-100%. The CAST may thus be useful as a novel in vitro test system in order to screen for possible hypersensitive reactions to NSAIDs with both reliability and safety.

Author information

Author/s: Kubota, Y (Y); Imayama, S (S); Toshitani, A (A); Miyahara, H (H); Tanahashi, T (T); Uemura, Y (Y); Koga, T (T); Sugawara, N (N); Kurimoto, F (F); Hata, K (K);

Affiliation: Department of Dermatology, School of Medicine, Fukuoka University, Japan.

Journal and publication information

Publication Type: Journal Article

Journal: International archives of allergy and immunology (Int Arch Allergy Immunol), published in SWITZERLAND. (Language: eng)

Reference: 1997-Dec; vol 114 (issue 4) : pp 361-6

Dates: Created 1998/01/20; Completed 1998/01/20; Revised 2004/11/17;

PMID: 9414140, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

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